FFPE DNA faamau

Ffpe dna faʻamamaina pusa o loʻo faʻaalia ai ata

Faʻamatalaga puʻupuʻu:

O le ato e faʻaogaina ai se siosiomaga faʻaʻaisa o le faʻaʻaisa o le faʻamamaina o le sili-lelei o le sili-lelei o faʻataʻitaʻiga mai le paraffin-octoding e mafua mai i le masani cross-fesoʻotaʻi.
O le faʻataʻitaʻiga e fefiloi ma le sela lysis buffer e vavaeina sela ma faʻaalia le dna. O le faʻataʻitaʻiga o loʻo fefiloi ma le sela lysis buffer e talepe sela ma faʻaalia le DNA. O le DNA e puʻeina DNA ua puʻeina e le maneta bedaptortics e fai ai se manava-dna faigata. O le lapisi-dna o loʻo fesiitai i totonu o lapisi-dna o loʻo fesiitai e fufulu ai le buffer e aveese ai le tele o mea leaga. Ma le mea mulimuli, o le DNA o loʻo faʻamamaina ma toe maua e ala i le faʻaliliu atu o se mea e fiafia ai.

FAAFESOOTAI MATOU

Faamatalaga auiliiliga

Oloa Tags

Faʻataʻitaʻiga

Ffpe vaega, ffpe poloka, faʻataʻitaʻiga i le faʻatulagaina o fofo e pei o faʻamatalaga masani

Vaega

Faʻataʻitaʻiga	Bfmp12r1	Bfmp12r16	Bfmp12r	Vaega
Afifiina	1T40t / pusa	16t16t / pusa	32t32T / pusa
Porokase k	400μl x 2	320 Auala	640μl	Filifiliga Filifiliga (Vavae ese)
Rnase a	80ëal	32μl	64μl	Enzyme fofo (Vavae ese)
Buffer fa	12ml	5ml	10ml	Sili onalgine fofo
Buffer fl	I le fasefulu 6-lelei sauni. pulufana 40 PCs.	96 Ia Muai-teuina ipu 2 pcs	96 Ia Muai-teuina ipu 2 PCs.	Malosi le faafitia ma tris buffer
Beffer wa				Sili onalgine fofo
Buffer wb				Maualalo masima fofo
Buffer de				Maualalo masima fofo
Magnetic Beds				Hydroxy magnetic Bead Fofo
Tagata Tusi Lesona	1	1	1