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Magleare Oryza Savata L. Praymic DNA faamau
Faatomuaga puupuu
O lenei pusa o loʻo lagolagoina se faʻapitoa atiaʻe ma le sili ona lelei faiga faʻavae ma maneta o loʻo fusia faʻapitoa i le dna, a o ave vave ma le le mautonu nuccharbrials ma le le mautonu i laʻau. E matua talafeagai mo le aveeseina o le damemic dna mai le totoina o le lag. I le lagolagoina o le faʻaaogaina o le lapoʻa lapoʻa maneta bedetic ackic acid sticractor, e matua talafeagai mo otometi le faʻaaogaina o le tele o faʻataʻitaʻiga tele. O le Destomic Denmic Dna e maualuga le mama ma lelei lelei, ma e mafai ona faʻaaogaina lautele i lalo o le pcrstream PCR / QPCR, NGS ma isi suʻesuʻega suʻesuʻega.
Foliga o oloa
◆ saogalemu ma le leai se mea oona: leai se mea e manaʻomia ai le oona oona o loʻo toe fai e pei o le phnol / chloroform
◆ otometi maualuga-alataʻuga: Faʻatulagaina ma Bigfish Nuncoic Acad Expracractor, e mafai ona faia le maualuga-alatsict Extractiction ma e talafeagai mo le tele o faʻataʻitaʻiga tele
◆ Maualuga Mama ma Lelei Lelei: O le faʻaopopoina o oloa e maualuga le amio mama ma mafai ona faʻaaoga mo le maualalo o le au, chip chibridization ma isi faʻataʻitaʻiga.
Taualumaga mo le aveeseina
Sampling: fou faʻataʻitaʻiga e uiga i le 100 mg poʻo le matutu mamafa faʻataʻitaʻiga e uiga i le 30 mg
Olo: O loʻo olo atoa ma le suavai nitrogen poʻo se ata
Digings: 65 ℃ Fanaʻele vai mafanafana
I luga o le masini: centrifrifle le supernatant ma faʻaopopo i le ipu mo le faʻaopoopoina
Meafaigaluega fetuutuunai
Bigfish Bfex-32 / BFFAX-32E / BFFAX-96E
Faapitoa o oloa
| Igoa o oloa | Pusi. Leai. | Afifiina |
| MakaaMamaOryza Satava L.Cenmic DNA faamau(ptoe tumu afifi) | Bfmp23R | 32t |
| MakaaMamaOryza Satava L.Cenmic Dna Faamāina pusa (muamua-tumu afifi) | Bfmp23R96 | 96T |
| Fomai po (pfaanatinati) | Bfrd007 | 1ml /faagaau (10mg / ml) |
| Rnase a(pfaanatinati) | Bfrd017 | 1ml /faagaau (10mg / ml) |
Faalauaiteleina tulaga