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E mafai ona vaevaeina meaola manu i le epithelial tissues, connective tissues, muscular tissues ma neural tissues e tusa ai ma o latou tupuaga, morphology, fausaga ma uiga masani o galuega, lea e fesoʻotaʻi ma felagolagomai i vaega eseese e fausia ai le tele o totoga ma faiga o manu ina ia faʻamaeʻaina ai le tele o gaioiga physiological.
Epithelial tissue: e aofia ai le tele o sela epithelial vavalalata vavalalata ma se vaega itiiti o sela interstitial o le fausaga e pei o le membrane, e masani ona ufiufi i luga o le tino o manu ma le tino o tubes eseese, cavities, capsules ma le pito i totonu o nisi totoga. Epithelial tissue o loʻo i ai galuega o le puipuiga, faʻamalo, faʻafefe ma faʻafefe.
O mea faʻafesoʻotaʻi: E aofia ai sela ma se aofaʻi tele o matrix intercellular. O le mea fa'afeso'ota'i e gaosia e le mesoderm o le tele lea o le tufatufaina ma le tele o ituaiga o meaola manu, e aofia ai le ga'o feso'ota'iga, mea fa'afeso'ota'i mafiafia, mea fa'afeso'ota'i reticular, mea fa'apipi'i, mea ponaivi, tino ma isi. E i ai galuega o le lagolago, fesoʻotaʻiga, puipuiga, puipuiga, toe faʻaleleia ma felauaiga.
Muscle tissue: e aofia ai sela maso e iai le malosi e fa'akonekarate ai. O foliga o sela maso e pei o le alava, o lea e taʻua ai foʻi le alava maso. O le galuega autu a le alava maso o le fa'akonekarate ma fa'atupu le gaioiina o maso. E tusa ai ma le morphology ma le fausaga o sela maso ma galuega eseese, o maso maso e mafai ona vaevaeina i musele skeletal (transverse muso), maso lamolemole ma muso cardiac.
Aano o le neura: aano e fausia i sela neura ma sela glial. O sela o neura o vaega ia o le morphological ma le galue o le tino ma e iai le malosi e lagona ai faʻamalosi i totonu ma fafo ma faʻatautaia lagona i totonu o le tino.
Oloa Bigfish
O le oloa e faʻaaogaina se faiga faʻapipiʻi faʻapitoa e faʻatupuina faʻapitoa ma sili ona lelei ma faʻamau maneta o loʻo fusifusia faʻapitoa le DNA, lea e mafai ona vave fusifusia ma faʻapipiʻi, vavae ese ma faʻamamā nucleic acids. E fetaui lelei mo le faʻaleleia lelei ma le faʻamamaina o le genomic DNA mai ituaiga uma o aano o manu ma totoga i totonu (e aofia ai meaola o le gataifale), ma e mafai ona sili atu ona aveese ituaiga uma o polotini, gaʻo ma isi mea faʻalaʻau ma isi mea leaga. E mafai ona faʻaaogaina ma leI'a teleMetotia Bead Metotia Nucleic Acid Extractor, lea e fetaui lelei mo le faʻaogaina otometi o lapoʻa faʻataʻitaʻiga tetele. O oloa ua maua mai le nucleic acid e maualuga le mama ma le lelei, ma e mafai ona faʻaaogaina lautele i lalo ifo o le PCR/qPCR, NGS, Southern hybridization ma isi suʻesuʻega faʻataʻitaʻiga.
Vaega:
Tele ituaiga o fa'ata'ita'iga: genomic DNA e mafai ona maua sa'o mai ituaiga uma o fa'ata'ita'iga a le manu
Saogalemu ma le oona: o le reagent e le o iai ni solvents oona e pei o le phenol, chloroform, ma isi, ma le maualuga o le saogalemu.
Otometi: fetaui ma le Bigfish Nucleic Acid Extractor e mafai ona faʻaogaina mo le faʻaogaina maualuga, aemaise lava mo le faʻataʻitaʻiga lapoʻa tele
Ole mama maualuga: e mafai ona faʻaaogaina mo suʻesuʻega o meaola mole mole, pei ole PCR, faʻamaʻi faʻamaʻi ma faʻapipiʻi saʻo.
Meafaigaluega Fa'atatau: BFEX-32/BFEX-32E/BFEX-96E
Fa'agasologa o le aveeseina:
Fa'ata'ita'iga: 25-30mg o aano o manu
Ole oloina: olo oloina vai, olo olo po'o sele
Digestion: 56 ℃ fa'afefete ta'ele mafanafana
I luga o le laupapa: centrifugation e aveese ai le supernatant, ma faaopoopo i le ipu loloto-vaivai mo le aveeseina o luga o le laupapa.
Faʻamatalaga faʻataʻitaʻiga: 30mg o faʻataʻitaʻiga o mea mai vaega eseese o isumu na ave ma faʻapipiʻiina DNA ma faʻamamaina i le BFMP01R e tusa ai ma faʻatonuga. O faʻaiʻuga faʻataʻitaʻiga na faʻaalia ai o le pusa BFMP01R o loʻo i ai se fua faʻatatau lelei.
Taimi meli: Iul-17-2025
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